TNS Essential Serum and Anti-Aging: A complete guide to perfect skin

TNS Essential Serum and Anti-Aging: A complete guide to perfect skin

To help improve the appearance of fine lines, wrinkles, skin tone and texture, TNS Essential serum blends growth factors that the body naturally secretes to stabilize the skins suffer.

One of the main reasons to use TNS serum is to defend the skin against enzymes called matrix metalloproteinases (MMPs). These enzymes are harmful causing damage to the look and feel of the skin. MMP enzymes are always present in the body and break down collagen fibers as women age or from outside environmental factors like catching rays or getting your bronze on in the sun.

There a several simple ways collagen can promote youthfully firm looking skin.

What are free radicals?

Free radicals are oxygen-containing molecules with an uneven number of electrons so they can easily cause damage to the body by way of chemical reactions throughout parts of the body including skin. Oxidative stress is an imbalance that leads to fine lines, wrinkles and aging in general. This is why antioxidants are so important to skin care and anti-aging.

What is skin collagen?

Collagen is the most abundant protein in the body and the skin makes up a large majority of that abundant share. Skin collagen is a huge network of fibers and as women age, these fibers begin to break down over time. Other negative factors that impacts the skin are UV rays from the sun, spreading of free radicals, pollution and cigarette smoke. when compounding damage is done without proper skin care, fine lines and wrinkles begin to rear their ugly face.

It’s very important to protect the skin’s collagen fibers to slow down the breakdown process.

How important in sunscreen?

Sun block is the most effective way women (and men) can protect against harmful UV exposure to stop MMP damage overtime. Dermatology grade sunscreen like SkinMedica can not only stop harmful MMPs from hurting the skin but the serum can also hydrate and prevent free radical oxidation. Aging and MMPs go hand-in-hand meaning with age brings more MMPs. By using sunscreen, women can protect their skin from UVA and UVB rays.

  • Ultraviolet A (UVA) is impacts skin aging.
  • Ultraviolet B (UVB) impacts skin burning.

How can women protect their skin?

There are several ways to reduce skin damage and improve the structural integrity of the skin so we recommend using a multi-step approach to give skin the level of care it desires and thrives on. Skin type will be the main determinate for our providers to help you chose which product is right for you. The top of the line skin care serums include the following ingredients – antioxidants, collagen boosters, exfoliants, sun protection, and most importantly growth factors.

What are skin care growth factors?

Growth factors are not growth hormones, they are naturally occurring proteins that move throughout the body. These growth factors are naturally occurring substances made by the skin cells that helps the body repair damaged skin cells thus slowing the signs of aging and impact of free radicals. Growth factors primary help grow fresh collagen and elastin help skin to remain firm and elastic. Similar to IGF-1 and growth hormone, these proteins tell skin cells to regrow new skin and heal existing the layers of skin. Reference

There are three layers of skin – the epidermis, dermis, and hypodermis – and growth factor proteins are present in all three layers. Each layer of skin works together to regrow and heal itself but with age bring compounding damage to the skin.

The epidermis is the outer visible layer of skin.

The Dermis is fibrous layer of the skin just below the epidermis. The dermis store mostly collagen and elastin fibers that help the skin stretch.

The dermis contains nerves, blood vessels, nerves, hair follicles, and glands that allow the skin to sweat and hold scent.

Elastin fibers are key proteins and (as you might expect) they are highly elastic and help connective tissues throughout the body to resume normal shape after stretching or contracting. Elastin also helps the skin return to its original position when it is poked or pinched.

The natural cell turnover process begins in the basal layer of the epidermis. Key cellular components all play a role in the health, and reproduction of all cells within the body.

As women age, the body has trouble getting rid of excess free radicals because the production of mitochondria that provides the skin cells with energy and rids the body of free radical toxins begins to slow down year over year.

In younger women, free radicals actually serve a positive purpose in the development of muscles, bones, and the nervous system. Older women don’t need that help.

Once women become fully developed the fibroblast cells that produce collagen and elastin stop reproduction so the only way women can trigger new growth occurs through skin care supplements like SkinMedica or through common wound healing processes (think micro needling).

How does skin change over time?

The aging process begins at the suffer of the skin (the dermis) where collagen and elastin levels begin to decrease over time. Free radicals begin to harden skin fibers by stiffing up through cross-linking resulting in a lack of firm cushioning on the skin layers above.

Blood flow is critical for the nutrition of healthy skin. Aging impacts the deeper layers of skin leading to dry skin and wrinkles.

As we learn throughout of Men’s Health blog, hormonal changes impact both women and men at the age of 40 and above. The varies stages of life from puberty to pregnancy to menopause causes drastic hormonal fluctuation within the body. When estrogen levels begin to decrease during menopause the production of glycosaminoglycans drops. This is bad because glycosaminoglycans helps the skin glow from deep within the dermis. These cells keep the skin with lubrication, suppleness, and thickness.

Epidermal Changes

Aging impacts organ performance due to the reduction of cell recreation and other physiological changes from hormonal imbalance to general wear and tear. All of these factors directly influence the visible appearance of the skin.

Skin is naturally supposed to shed and recreate fresh skin cells but this slows down over time leading to a dull, uneven, and rougher looking texture. The decrease in cell recreation also leads to dry skin. The shedding process (keratinization) helps with the creation of key lipids within the top layers of the skin.

Lipids are natural fats present in the skin. These fats serve an important purpose keeping the strength of the skin’s outer protective layer intact and aid in the repairing process. This same layer holds moisture protecting the skin from damage and keeps airborne dirt from getting under the skin.

  • Cholesterol helps the skins outer barrier recover and improve elasticity. Low cholesterol commonly impairs the look of skin causing the outer layer to look older. Skin cholesterol is different than the cholesterol floating in the bloodstream. All organs in the body use cholesterol to lubricate and run smoothly – skin is in that category.
  • Ceramides helps the skin retain moisture keeping hydration steady fending off dry skin.
  • Fatty acids that are abundant in healthy skin helps to maintain the skin’s lipid balance.

Lipid imbalance causes the skin to become more susceptible and sensitive to any sort of outside environmental factors. The lack of skin cell regeneration also contributes to spotty skin pigmentation changing the natural skin tone creating uneven natural color.

In order to regain a healthier looking complexion, women should look at replenishing their skin with missing lipids the skin thrives on.

Aging skin is more prone to irritation and loss in hydration is it is important to counter those effects with topical treatment, but not just any topical treatment. This is why we use dermatologist grade SkinMedica for our ladies.

Many studies have proven that the most effective topical products include formulas that mirror the skins own lipid composition – like 1:2:1 ceramides to cholesterol to fatty acids ratio.

This lipid ratio helps significantly improve the appearance and signs of aging. These lipid formulas help reinforce the outer layers of skin regardless of cosmetic treatments, which is essential for optimal healing and results when considering cosmetic therapy.

What does personalize skin care mean?

All skin behaves differently and one cannot define what maturing skin actually entails meaning all women at varying ages are impacted by activity levels, heredity factors, product use, and general behavior (work, life, etc).

One constant factor in how we define maturing skin centers around a decrease in skin cell recreation, suppleness, moisture, firmness, and elasticity. Aging or maturing skin will appear dull and show signs of aging that include fine lines, wrinkles, uneven texture and hyperpigmentation. While it’s nearly impossible to stop all signs of aging, our providers can help influence some of the skin changes through SkinMedica serum intervention. There is really no guess work in what SkinMedica includes in their products because these products have been trusted by dermatologist and their patients for decades. The ingredients have proven with evidence to positively influence the signs of aging skin.

The obvious goal for all women and our providers is to keep the skin as youthfully vibrant as long as possible year over year. Another important goal for our patients is to promote healthy skin cell regeneration, hydrate, nourishment, and protection. There are tons of awesome skin care technologies and products out in the market. Although we would like you to consider us a trusted advisor, we urge you to do your own due diligence on products by learning the ingredients and facts behind the product. Luckily, we have included the most popular opinions in this article to save you some time.

Luckily, aging skin is a by-product of life that all women and men must deal with. It certainly something all people should take seriously.

Peptides help support skin:

Peptides one of the most popular ingredients used anti-aging field of skincare because they strengthen skin and rejuvenate damage deep within the epidermis.

What are peptides?

Peptides are short chains of amino acids, which are building blocks for proteins inside each layer of the skin (collagen, elastin and keratin). All of these proteins are critical to keep the texture strong and smooth. When there are not enough peptides in the skin wrinkles begin to show up firming up the top most layer of skin making women look older than they should.

Peptides tell skin cells to start specific functions like requesting the regrowth of collagen, smooths elastin fibers and hydrates deep fibers, which improves the look and feel of the skin. Without peptides, wrinkles will appear, nails will become brittle and hair will become dry, weak, and thin.

Outside of skin structure and function, peptides also play a key role in bodily function as they are biologic messengers (think enzymes, hormones, energy sources) that play a role in telling the body to secrete hormones like estrogen or tell the gut to release enzymes to break down toxins.

How Peptides in Your Skin Care Work

While peptides are ever present within the body, as women get older the body’s natural ability to reproduce new collagen decreases by 1% per year after the age of 30. This decrease in regrowth leads to older looking skin. Reference

The body uses peptides as signaler to tell the body to heal damage within the body. If the skin is aging prematurely or red from sun damage, peptides signal the skin cells to start cooking up a fresh batch of collagen to repair the impairments. Peptides also signal the production of hyaluronic acid to restore a healthy skin barrier making the skin appear supple and refreshed.

When applying peptide infused moisturizer or serum onto the skin, the body thinks there is a skin wound or injury in need of repair so the serum stimulates collagen production.

It’s important to note that not all peptides are created equal. While there are all sorts of peptides, only a select few help the skin look great.

  • Carrier peptide help deliver minerals to skin cells to boost the production of collagen.
  • Enzyme inhibitor peptides slow down the natural breakdown of collagen.
  • Signal peptides ask different parts of the skin to promote collagen, elastin and keratin.
  • Neurotransmitter peptides freeze muscles to remove expression lines and smooth wrinkles. Note: these peptides are not the same as Botox.


What’s Inside TNS Essential Serum?

There are two chambers side-by-side in each bottle of TNS serum.

Chamber 1: TNS Recovery Complex®

Human Fibroblast Conditioned Media (TNS®): A mixture of growth factors, cytokines, antioxidants and matrix proteins. 

What are growth factors?

Growth factors are messenger proteins that are inherent in our bodies and play a role in maintaining healthy skin.

What is APS Corrective Complex?

A patented mixture of antioxidants, peptides and other innovative ingredients that in combination improves the overall appearance of skin.

Chamber 2: APS Corrective Complex

Palmitoyl Tripeptide-5

Highly bioactive, deeply skin penetrating peptide (palmitoyl tripeptide-5). Has been found in-vitro studies to activate tissue growth factor (TGF-beta). TGF-beta is known to stimulate collagen synthesis in the skin. The peptide content is 900-1300 ppm (approx. 1g peptide/1kg solution). Dissolved in glycerin & water. Preservative-free. Clear liquid, no odor. Easily water-soluble.


  • In-vitro tests showed that tripeptide has better collagen building capacity than TGF-beta.
  • Has been found to improve significantly the appearance of any type of wrinkles
  • Has good skin firming and moisturizing properties.
  • May be able to reduce appearance of stretch marks. In clinical study with 60 volunteers a significant reduction of the appearance of wrinkles was shown after use of 84 days.
  • Could be used as alternate method to collagen injections used in lip products.

Use: Add as is at the end of formulas. Typcial use level: 1 – 3%. For external use only.

Applications: Anti-aging & anti-wrinkle products, pre/after sun lotions, hydrating skin care treatments, lip products, eye wrinkle treatment.

Manufacture: Tripeptide-5 is produced synthetically from amino acids with a unique sequence to mimic the human body’s own mechanism to produce collagen via TGF-beta.

Animal Testing: Not animal tested

GMO: GMO free but not certified

Vegan: Does not contain animal-derived components


Hydrolyzed Silk (Sericin)

Description: Unique glycoprotein isolated from natural silk produced by the silk worm Bombyx mori. Hydrolzyed (fragmented) for better solubility and skin-penetration. Has strong affinity to keratin and forms a natural semi-occlusive, protecting anti-wrinkle film on the skin surface imparting an immediate, long lasting, smooth, silky feeling. Amber aqueous liquid. Water-soluble.

Properties: Hydrolyzed sericin provides excellent film-forming, conditioning, and anti-wrinkle properties. It reduces significantly transepidermal water loss and skin irritation. Smoothing and moisturizing activities that persist even after washing leaving the skin or scalp silky and smooth.

Applications: Anti-aging and anti-wrinkle creams lotions, and serums. All kinds of conditioning hair care products.

Manufacture: Extracted and purified from the silk worm Bombyx mori. The protein is then hydrolyzed.

Animal Testing: Not animal tested

GMO: GMO-free (does not contain plant-derived materials)

Vegan: No


Dipalmitoyl Hydroxyproline

Description: Plant-derived lipid agent consisting of hydroxyproline (natural amino acid essential for skin collagen synthesis) and palmitic acid (natural fatty acid). Has naturally high affinity for skin proteins. White granular powder, faint odor. Insoluble in water, alcohol, acetone, but miscible in propylene glycol or oils.


  • Long-lasting firming and moisturizing effects. Has been shown to reduce the appearance of wrinkles and provide volume, suppleness, and a former tone.
  • DH has been found to stimulate the synthesis of collagen fibers and inhibit age-promoting elastase enzymes in-vitro.
  • Contains a hydroxyproline vehicle that moisturizes, strengthens, and rejuvenates the skin.
  • Effective for lip plumping.

Applications: Anti-aging, anti-wrinkle & anti-stretch mark creams & lotions, firming / toning products, moisturizing and lip treatment formulations.

Manufacture: SkinLift DPHP is produced by combining palmitic acid with the amino acid hydroyproline.

Animal Testing: Not animal tested

GMO: GMO free but not certified

Vegan: Does not contain animal-derived components

Ergothioneine (EGT) 

Antioxidants that help prevent free radical damage. An antioxidant with free radical scavenging properties. Reference

L-ergothioneine (EGT) is a stable antioxidant found in food plants as well as in animal tissue undergoing relatively high levels of oxidative stress. Idebenone is a stable analog of the antioxidant coenzyme Q(10). All are potent antioxidants found in skincare products, but their relative potencies are not well described. AIMS: To establish the physiological relevance of EGT by examining transcription of the EGT transporter gene OCTN-1 and production of the receptor protein in skin fibroblasts. In addition, to compare the inhibition of lipid peroxide formation by coenzyme Q(10) and EGT. Furthermore, to compare the peroxide-scavenging abilities of EGT and idebenone in both simple solution and in cell cultures exposed to ultraviolet A (UVA).

Ubiquinone (Coenzyme Q10)

An antioxidant that helps to counteract free radical damage and works synergistically with vitamin E.

Description: Vitamin-like molecule (ubiquinone, ubidecarenone) obtained from plant material. Q10 is encapsulated in nano-sized liposomes consisting of a phospholipid double nano-emulsion with vitamin E and vitamin C (ascorbyl tetraisopalmitate) to maintain activity, enhance skin penetration and potency. Accompanying vitamin E and vitamin C are needed to activate coenzyme to its active state. The liposomes have an average particle size of about 50nm. Can be easily incorporated into aqueous formulas. Activity 6.8-7.3% Q10 (liposome-encapsulation largely reduces the amount of Q10 required for effectiveness as compared to non-encapsulated pure Q10). Preserved with 0.1% sodium benzoate. Orange-opaque liquid, faint odor.


  • Excellent antioxidant due to vitamin E and vitamin C.
  • Can significantly reduce the appearance of aged skin and wrinkles. Has been found to improve appearance of aged skin.
  • Potent moisturizer as liposomes can penetrate into skin minimizing water-loss.
  • Ideal to fight against scalp and hair aging but also to reinforce and revitalize hair.

Applications: Anti-aging & anti-wrinkle products, hair treatments/hair serums, pre/after sun lotions, hydrating / rejuvenating & moisturizing skin care products.

Manufacture: Coenzyme Q10 is produced through microbial and/or yeast fermentation. It is then formulated into a nanoemulsion combined with vitamin E and vitamin C

Animal Testing: Not animal tested

GMO: GMO free but not certified

Vegan: Does not contain animal-derived components

Camellia Oleifera (Green Tea) Leaf Extract 

A natural antioxidant rich in polyphenols.

 Tetrahexyldecyl Ascorbate A stable form of ascorbic acid (Vitamin C), a proven antioxidant.

Description: Highly stable, oil-soluble vitamin C ester. Has excellent skin penetration and as a result offers increased cell protection against UV-B. The skin cell viability is increased up to 30% when compared with l-ascorbic acid. Clear yellowish liquid. Oil-soluble, water-insoluble.


  • Can improve appearance of aged and fragile skin.
  • Widely used as add-on ingredient in skin-lightening products to correct hyperpigmentation and age spots.
  • Antioxidant effect can be increased by combining L-ascorbic acid with L-ascorbyl palmitate and/or vitamin E.
  • Does not give finished formulas a yellowish tint like l-ascorbic acid.

Applications: Lotions, gels, creams, sun care & after sun products, makeup, lip products.

Manufacture: Tetrahexyldecyl ascorbate is produced by adding hydrocarbon chains (oily substances) such as trihexyl hexadecane to ascorbic acid whereby ascorbic acid becomes stabilized and oil-soluble.

Animal Testing: Not animal tested

GMO: GMO free (does not contain plant-derived components)

Vegan: Does not contain animal-derived components

Tocopheryl Acetate 

A form of vitamin E, an antioxidant and free-radical scavenger.

Description: Water-insoluble vitamin E formed by esterification of acetic acid & tocopherol. Purity >98%. pH 6-8. Activity: 1000 IU per 1 g solution (about 1 ml). Clear viscous yellow liquid, faint odor. Soluble in alcohol & oil, water insoluble.


  • Provides excellent antioxidant activity on the skin but less so in cosmetic products since enzymes need first to cleave off acetate to form active vitamin E.
  • Has moisturizing, soothing, and calming properties on the skin.
  • Can improve the appearance of aged skin making it look visibly younger.

Applications: All kinds of skin care products including sun care & makeup products.

Manufacture: Dl-alpha tocopheryl acetate is produced synthetically by the condensation of 2,3,5-trimethylhydroquinone with isophytol in an inert solvent (e.g. benzene or hexane) with a catalyst such as zinc chloride. The resulating alpha tocopherol is then treated with acetic anhydride to give tocopheryl acetate.

Animal Testing: Not animal tested

GMO: GMO free (does not contain plant-derived components)

Vegan: Does not contain animal-derived components

Rubus Fruticosus (Blackberry) Leaf Extract 

A natural antioxidant rich in polyphenols

Extract of blackberry leaf (Rubus fruticosus) is a common traditional medicine which recently has been discovered to benefit the suppleness and smoothness of human skin.  According to a recent study, it boosts the production of collagen and elastin, two compounds which are responsible for skin’s elasticity and firmness.

How does it work? 

According to studies, blackberry leaf extract is made up of many functional ingredients which work together to inhibit enzymes in the skin called matrix metalloproteinases (MMPs). MMPs, which are fed by exposure to UVA radiation, are an enemy to youthful skin because they break down both collagen and elastin.

Researchers have found that blackberry leaf extract inhibits the activity of these enzymes, enabling skin to retain its elasticity and smoothness for longer.  It also contains antioxidants similar to those found in green tea.


Saccharomyces Ferment Lysate Filtrate 

A bio-optimized yeast extract that helps prevent free radical damage.

Saccharomyces lysate is a probiotic yeast obtained by fermentation. Its by-products, known as postbiotics, can help normalize skin’s microbiome by curbing the proliferation of microorganisms that can trigger signs of impaired skin.

Saccharomyces Lysate is a group of fungi that ferment sugars, and a source of beta-glucan, which is a good antioxidant.


Brightens skin.

Description: Alpha-arbutin (4-hydroxyphenyl-D-lucopyranoside) is a synthetic and functional active ingredient for skin lightening. Biosynthetic ingredient, derived from bearberry. Crystalline, white powder. Water-soluble. Stable in the pH range from 3.5-6.5.


  • Promotes skin lightening and an even skin tone on all skin types.
  • Reduces the degree of skin tanning after UV exposure.
  • Helps to minimize the appearance of liver spots.

Applications: All kinds of skin-lightening products including lotions, creams, makeup products.

Manufacture: Alpha-arbutin is produced by reacting hydroquinone and dextrine (a sugar) with a glycosylating enzyme.

Animal Testing: Not animal tested

GMO: GMO-free but not certified

Vegan: Does not contain animal-derived components

Benefits of Arbutin

Arbutin has sun protection properties reducing the degree of skin darkening after sun exposure by blocking the production of tyrosinase.

Gentle on skin: While other skin brightening agents can dry and irritate the skin, arbutin is less irritating.

Improves uneven complexion: Since arbutin inhibits tyrosinase, an enzyme that helps with melanin production, it can prevent dark spots or even help fade them, leading to a more even complexion.

Safe to use with other skincare products: There are no known negative interactions between arbutin and other skincare ingredients.

Twice daily application: For maximum results, arbutin can be used both in the morning and at night.

Effective at fading scars: Much like its ability to fade dark spots, arbutin is also great at fading those red-purple acne scars that stick around long after a zit is gone.

Brightens skin: As explained above, arbutin prohibits the formation of dark spots, offering a brightening effect on the skin. Reference

Hyaluronic Filling Spheres Dehydrated filling spheres, containing hyaluronic acid, help trap water on the surface of the skin like a sponge.

HFS has the ability of dehydrated and cross-linked spheres to penetrate the upper layers of the epidermis and to absorb the water that evaporates from the deep dermis. 


Human Fibroblast Conditioned Media, Water/Aqua/Eau, Cetyl Ethylhexanoate, Olea Europaea (Olive) Fruit Unsaponifiables, Alpha-Arbutin, Isoceteth-20, Arachidyl Alcohol, Glycerin, Ethoxydiglycol, Butyrospermum Parkii (Shea) Butter, Dimethicone, Tetrahexyldecyl Ascorbate, Dipalmitoyl Hydroxyproline, Palmitoyl Tripeptide-5, Ergothioneine, Hydrolyzed Sericin, Phospholipids, Ubiquinone, Rubus Fruticosus (Blackberry) Leaf Extract, Saccharomyces Ferment Lysate Filtrate, Aminobutyric Acid, Phytosterols, Tocopherol, Tocotrienols, Squalene, Oryza Sativa (Rice) Bran Wax (Oryza Sativa Cera), Sodium Hyaluronate, Tocopheryl Acetate, Camellia Oleifera Leaf Extract, Ethylhexyl Palmitate, Silica Dimethyl Silylate, Polyacrylate-13, Polyisobutene, Polysorbate 20, Behenyl Alcohol, Arachidyl Glucoside, Cetearyl Alcohol, Steareth-10, Steareth-20, Butylene Glycol, Maltodextrin, C12-15 Alkyl Benzoate, Xanthan Gum, Acrylates/C10-30 Alkyl Acrylate Crosspolymer, Aminomethyl Propanol, Disodium EDTA, Caprylyl Glycol, Caprylhydroxamic Acid, Ethylhexylglycerin, Phenoxyethanol, Potassium Sorbate, Parfum/Fragrance, Hydroxycitronellal, Linalool, Coumarin, Alpha-Isomethyl Ionone, Geraniol, Isoeugenol

When should I use this product in my daily skin care routine?

TNS Essential Serum® should be applied after cleansing and toning the skin and prior to applying any other products, including sunscreens. Note: TNS Recovery Complex® is in one chamber of the TNS Essential Serum® pump, you do not need to incorporate TNS Recovery Complex® in your daily routine.

How long to use?

You should use TNS Essential Serum® as long as you wish to improve the overall appearance of your skin. Our clinical studies show that initial results occur in 30 days with optimal results occurring in 90 days. For best results, you should use twice daily and incorporate as part of your daily skin care routine.

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